Page 17 - Aquauclature Research and Studies (01)

AQUAUCLATURE
7
Research and Studies 1 (1): 15-01, 2026 page of 193

Nutri- Fishmeal SBM Untreated Soaked Autoclaved
ent CSGM CSGM CSGM
Va- 1.99±0.02 1.86±0.01
line 1.67±0.01 1.70±0.01 1.72±0.00
Anti-nutrional factor
b
a
b
Tan- 2.77±0.12 0.80±0.03 0.78±0.00
1
nins
a
Phyti 0.91±0.03 0.33±0.05 0.26±0.00
b
b
c
2
acid
c
a
b
Sapo- 0.33±0.01 0.21±0.00 0.16±.01
3
nins
a
b
c
Tryp- 6.45±0.61 4.13±0.38 1.15±0.07
sin in-
hibi-
4
tor
1 As tannic acid equivalent per mg/g sample. As mg/g. As dysgenic equiva-
2
3
4
lent per mg/g sample As Milligram of pure trypsin inhibited per gram of
sample.
2.3. Water quality
Every morning, the measurement of the temperature and dissolved oxy-
gen was taken while measurement of the ammonia, pH and nitrite was done
once a week. The water temperature, dissolved oxygen, ammonia, nitrite, and
−1
pH ranged 25–28°C, 5.8–6.3 mg L , < 0.5 mg, < 0.1 mg L −1 , and 7.0–8.0,
respectively. There was a 50% water exchange for each aquarium at every 3-
day intervals.
2.4. Chemical analysis
All ingredients, experimental diets, and freeze-dried faeces were finely
milled before they were analysed. Proximate analyses were conducted as de-
scribed in AOAC (1997). The essential amino acid concentrations of all the
ingredients (FM, CSGM, and SBM) and diets were established with high-
performance liquid chromatography (HPLC) as defined Strydom and Cohen
(1994). The nitrogen solubility in raw and processed CSGM samples was