Page 18 - Aquauclature Research and Studies (01)

AQUAUCLATURE
Research and Studies 1 (1): 15-01, 2026 page of 193
8

computed according to the method as described in Araba and Dale (1990).
ANFs in untreated and pre-treated CSGM (tannins, phytic acid, the activity
of trypsin inhibitors and saponins) were measured based on the methods de-
scribed in AOAC (1997),
Table 2: Composition of reference and test diets (% as fed basis)
Test Ingredient
Ingredient Reference Untreated Soaked Autoclaved
CGSM CGSM CGSM
Raw CSGM 0 29.3 0 0
Soaked 0 0 29.3 0
CSGM
Autoclaved 0 0 0 29.3
CSGM
Fishmeal 23.36 16.88 16.88 16.88
Soybean meal 35.7 24.75 24.75 24.75
Tapioca 30.04 20.82 20.82 20.82
starch
Crude palm 7.9 5.47 5.47 5.47
oil
Vitamin pre- 1 0.69 0.69 0.69
a
mix
Mineral pre- 1 0.69 0.69 0.69
b
mix
Chromic ox- 1 1 1 1
ide
Proximate composition
Dry matter 95.15±1.27 95.13±2.30 95.06±1.30 94.81±1.58
Ash 9.63±0.07 8.81±0.05 8.71±0.01 8.55±0.06
Protein 31.41±0.41 33.84±0.06 35.25±0.41 35.06±0.08
Lipid 7.76±0.01 5.97±0.08 6.97±0.20 6.69±0.15
Crude fibre 3.13±0.15 5.61±0.56 5.96±0.93 5.90±0.13
Carbohydrate 46.86±1.42 46.50±2.24 43.10±1.26 44.51±1.52