Page 19 - Aquauclature Research and Studies (01)

AQUAUCLATURE
Research and Studies 1 (1): 15-01, 2026 page of 193
9

Test Ingredient
Ingredient Reference Untreated Soaked Autoclaved
CGSM CGSM CGSM
Gross energy 17.11±0.19 17.63±0.42 18.09±0.17 17.85±0.03
-1
(kJ g )
Essential amino acids
Arginine 2.61±0.00 2.50±0.00 2.70±0.00 2.93±0.00
Histidine 1.55±0.01 0.91±0.00 0.77±0.00 0.89±0.00
Isoleucine 1.24±0.02 1.15±0.00 1.25±0.00 1.34±0.00
Leucine 2.14±0.00 2.33±0.00 2.43±0.00 2.73±0.00
Lysine 2.11±0.00 1.98±0.00 2.18±0.01 2.26±0.00
Methionine 1.21±0.01 1.01±0.00 1.04±0.00 1.11±0.01
Phenylalanine 1.34±0.00 1.32±0.00 1.42±0.00 1.49±0.00
Threonine 1.54±0.00 1.28±0.00 1.38±0.00 1.45±0.00
Valine 1.39±0.00 1.37±0.00 1.37±0.00 1.58±0.00

-1
a Vitamin premix (g kg premix): ascorbic acid, 45; myo-inositol, 5; choline chloride, 75;
niacin, 4.5; riboflavin, 1; pyridoxine, 1; thiamine mononitrate, 0.92; Ca-pantothenate, 3; ret-
inyl acetate, 0.6; cholecalciferol, 0.083; vitamin K menadione, 1.67; α-tocopheryl acetate
-1
(500 IU g ), 8; biotin, 0.02; folic acid, 0.09; vitamin B12, 0.001; cellulose, 845.11.
b Mineral premix (g kg−1 premix): KCL, 90; KI, 0.04; CaHPO4·2H2O, 500; NaCl, 40;
CuSO4·5H2O, 3; ZnSO4·7H2O, 4; CoSO4, 0.02; FeSO4·7H2O, 20; MnSO4·H2O, 3;
CaCo3, 215; MgOH, 124; Na2SeO3, 0.03; NaF, 1.

The chromic oxide in the diets and faeces was measured following
Furukawa and Tsukahara (1966). Sample was digested in a Kjeldatherm
block digestion unit until a white precipitate was obtained, and absorbance
was determined at 350 nm using an atomic absorption spectrophotometer
(Shimadzu, UV-1800).
The ADCs of nutrients and energy for the reference and test diets were
computed following Maynard et al. (1979) as described below.

ADC (%) = 100 × [1 − ( ) × ( )]

The ADC of dry matter was computed using the folllowing formula: