Page 47 - Aquauclature Research and Studies (01)

AQUAUCLATURE
Research and Studies 1 (1): 15-01, 2026 page of 193
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Table 1: Dietary carbon and nitrogen sources A,B,C,PM and RL levels .
Standard F/2 100 % 25 % 25 % 25 % 25 % 25 %
Fructose (A) - 75 %
Lactose (B) - 75 %
Glucose (C ) - 75 %
Poultry manure extract (PM) - 75 %
Rumen liquor (RL) - 75 %

Determination of algal biomass:
The biomass was chemically harvested (Fig 1. A) through flocculation
method described by (Sales&Abreu,2014) using NaOH (5 %) for 10
minutes.Then the water was siphon out and the flocculants were filtered
through plankton net with mesh20 micron. The wet biomass was then dried
o
in oven at 50C and weighed in g/L to determine algal growth in each medium
and stored for further using in biodiesel extraction.
Extraction and purification of total lipids:
The total lipids were exhaustively extracted by the addition of 200ml of
chloroform and methanol mixture (2:1 v/v) to a known weight of dried algal
biomass using Folch method (1957). Then the obtained total lipids were
weighed as g/g dry biomass for further oil(biofuel) and biodiesel extractions.
Biodiesel Extraction and purification methods (methylation)
The esterification method of Radwan (1978) was used, where the oil-
hexane extracted from the total fats was taken and placed in Falcon glass
tubes with 2 ml of benzene, then 1% methanolic sulfuric acid was added and
the bottles were tightly closed. It was placed in the oven at a temperature of
90°C for an hour and a half. After removing it from the oven, distilled water
was added to it and filtered well. Then, petroleum ether 60/80 was added to
it in stages until the biodiesel collected on the surface. We extracted it with a
syringe and placed it in the flask and left it until the chemicals evaporated and
the biodiesel remained.