Page 43 - Aquauclature Research and Studies (01)

AQUAUCLATURE
Research and Studies 1 (1): 15-01, 2026 page of 193
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Received: 5/8/2025 (4.25% and 3.82%) compared to the control group
Accepted: 12/10/2025 (3.52%). Accordingly, the red tilapia carcass achieved
Publish online:
15/01/2026 the maximum protein content in both B2 and B4 diets,
while the fat content was the highest in the control
group. Finally, the authors proposed the use of pome-
granate molasses as a new treatment to enhance the bio-
diesel content in terms of cost and residual biomass with
75% replacement of soybean meal in the diet (PM3,
PM4) and also achieved good results for improving the
culture of red tilapia.
Keywords: Nannochlorpsis, Biodiesel,Red tilapia,Aquaculture,Or-
ganic sources,Growth performance Biochical analysis

Introduction
Microalgae are among the most important live foods for rotifers, cladoc-
erans, and other zooplankton, as well as fish and shrimp larvae (Gallardo et
al., 1995). Nutritionally, many freshwater and marine microalgae play a dual
role in providing energy and essential nutrients, such as proteins, lipids, ca-
rotenoids, vitamins, amino acids, polyunsaturated fatty acids, and minerals,
which are essential for the healthy growth of aquatic organisms (Vimazal,
1995; Yamaguchi, 1997; Habib et al., 2003). Organic media were mainly
used in early experimental algal cultivation (Shelf et al., 1978). Optimizing
suitable culture media for large-scale microalgal biomass production is criti-
cal for industrial production. Microalgal culture media must be easy to pre-
pare (F/2), which is common in small-scale indoor cultivation, but are expen-
sive and difficult to prepare for outdoor mass cultivation, representing a major
obstacle to biofuel production. Therefore, agricultural fertilizers have been
commercially used as an alternative to F/2 culture medium (Sanchez-Saa-
vedra et al., 2005). Researchers have studied the growth of microalgae, while
others have investigated their biochemical components in response to the me-
dium (Feng et al., 2011; Martinez-Córdova et al., 2012). Nitrogen concentra-
tion, along with its source, plays a crucial role in enhancing lipid and biomass
production for biodiesel production (Vega et al., 2010). Bondioli et al. (2012)
reported that Nannochloropsis has a high lipid content (maximum 68.5%) and
a high lipid yield (maximum 271 ml/L/day), making it a good source for