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AQUAUCLATURE
Research and Studies 00 (0): 00-00, 2026
Doi:
Research Article Open Access
Potential Applications of Nannochloropsis oceanica
alga for biodiesel production and the de-lipidated
biomass for improving red tilapia aquaculture
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1
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Ali M. Abugrara , Heba S. El-Sayed *, Mohamed A. A. Zaki and Abd El-Aziz M.
Nour
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1- Lecturer, Marine re- Abstract: This work was conducted in two phases. The
sources department, Fac- first was to investigate the different effects of different
ulty of Natural Resources
and environmental sci- organic carbon sources [fructose (A), lactose (B), glu-
ences, Omar Al-Mokhtar cose (C)] and nitrogen sources [poultry manure extract
University, El-Beda, Libya. (PM) and rumen lysate (RL)] used in a 75:25% F/2 ratio
2- Associate professour in on maximizing biodiesel production from the marine
aquaculture division, Na- microalgae Nannochloropsis oceanica. The second was
tional Institute of oceanog-
raphy and fisheries, Qait to apply the residual lipid-free biomass as a feed additive
Bay, Alexandria, Egypt. in red tilapia culture. In the first phase, the biomass
3- Head of Animal and (DW), biodiesel contents, and biochemical composi-
Fish production depart- tions of N. oceanica were determined. The results
ment, Professour of fish showed variations in biomass and biochemical analysis
husbandary faculty of Agri-
culture (El-Shatby), Alex- in DW bases among treatments. However, A signifi-
andria University, egypt. cantly achieved the maximum DW, algal biomass, and
4- Professour of Animal protein content, while RL had the greatest lipid accumu-
and fish Nutrition, depart- lation and also achieved the highest biodiesel content. In
ment of Animal and Fish
production, faculty of Agri- the second phase, the secondary algal biomass (fat-free)
culture (El-Shatby), Alex- product from treatments B and PM was applied at four
andria University, egypt. rates (1, 2, 3, and 4) at concentrations of 25, 50, 75, and
*Corresponding author: 100%, respectively, as a substitute for soybean meal to
Ali M. Abugrara, Lecturer, feed red tilapia larvae compared to the control group.
Marine resources depart-
ment, Faculty of Natural The initial larval weight and length were 0.32 g and 1.9
Resources and environmen- cm, respectively, and were maintained for the 30-day
tal sciences, Omar Al- experimental period. After the end of the experimental
Mokhtar University, El- period, growth performance and biochemical analysis
Beda, Libya.
*ali.mahmoud@omu.edu.ly were evaluated. The results showed that the growth rate
(SGR) was significantly higher in PM4 compared to B1

