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AQUAUCLATURE
                        Research and Studies 00 (0): 00-00, 2026

                          Doi:
                         Research Article                                          Open Access

                                    Potential Applications of Nannochloropsis oceanica
                                     alga for biodiesel production and the de-lipidated
                                      biomass for improving red tilapia aquaculture
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                          Ali M. Abugrara , Heba S. El-Sayed *, Mohamed A. A. Zaki  and Abd El-Aziz M.
                                                          Nour
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                         1- Lecturer, Marine re-  Abstract: This work was conducted in two phases. The
                         sources department, Fac-  first was to investigate the different effects of different
                         ulty of Natural Resources
                         and environmental sci-  organic carbon sources [fructose (A), lactose (B), glu-
                         ences, Omar Al-Mokhtar   cose (C)] and nitrogen sources [poultry manure extract
                         University, El-Beda, Libya.   (PM) and rumen lysate (RL)] used in a 75:25% F/2 ratio
                         2- Associate professour in   on  maximizing  biodiesel  production  from  the  marine
                         aquaculture division, Na-  microalgae Nannochloropsis oceanica. The second was
                         tional Institute of oceanog-
                         raphy and fisheries, Qait   to apply the residual lipid-free biomass as a feed additive
                         Bay, Alexandria, Egypt.    in  red  tilapia  culture.  In  the  first  phase,  the  biomass
                         3- Head of Animal and   (DW),  biodiesel  contents,  and  biochemical  composi-
                         Fish production depart-  tions  of  N.  oceanica  were  determined.  The  results
                         ment, Professour of fish   showed variations in biomass and biochemical analysis
                         husbandary faculty of Agri-
                         culture (El-Shatby), Alex-  in  DW  bases  among  treatments.  However,  A  signifi-
                         andria University, egypt.    cantly achieved the maximum DW, algal biomass, and
                         4- Professour of Animal   protein content, while RL had the greatest lipid accumu-
                         and fish Nutrition, depart-  lation and also achieved the highest biodiesel content. In
                         ment of Animal and Fish
                         production, faculty of Agri-  the second phase, the secondary algal biomass (fat-free)
                         culture (El-Shatby), Alex-  product from treatments B and PM was applied at four
                         andria University, egypt.      rates (1, 2, 3, and 4) at concentrations of 25, 50, 75, and
                         *Corresponding author:    100%, respectively, as a substitute for soybean meal to
                         Ali M. Abugrara, Lecturer,   feed red tilapia larvae compared to the control group.
                         Marine resources depart-
                         ment, Faculty of Natural   The initial larval weight and length were 0.32 g and 1.9
                         Resources and environmen- cm, respectively, and were maintained for the 30-day
                         tal sciences, Omar Al-  experimental period. After the end of the experimental
                         Mokhtar University, El-  period,  growth performance  and  biochemical  analysis
                         Beda, Libya.
                         *ali.mahmoud@omu.edu.ly    were evaluated. The results showed that the growth rate
                                               (SGR) was significantly higher in PM4 compared to B1
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