Page 11 - Aquauclature Research and Studies (01)

AQUAUCLATURE
Research and Studies 00 (0): 00-00, 2026

Doi:
Research Article Open Access

Effects of Pre-Treating Carob Seed Germ Meal on
The Digestibility, and Plasma Amylase, Glucose and
Cholesterol in Red Tilapia
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Abdalbast H.I. Fadel , Mohd Salleh Kamarudin , Nicholas Romano , Mahdi Ebrahimi , Che
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Roos Saad , Anjas Asmara Samsudin
1 Department Marine Abstract: This study aimed to evaluate the effects of
Re-sources, Univer- pre-treatment of carob seed germ meal (CSGM) on the
sity of Omar Al-
Mukhtar, Al-Baida, digestibility and metabolic responses of red tilapia.
Libya Three treatments were used (raw CSGM, soaked
2 Department of Aqua-
culture, Universiti Pu- CSGM, and autoclaved CSGM, and incorporated at
tra Malaysia, 43400 30% into experimental diets compared with a reference
Serdang, Selangor, diet free of CSGM. Red tilapia were fed these diets for
Malaysia
3 Department of Vet- four weeks, after which fecal samples were collected to
erinary Preclinical determine apparent digestibility coefficients. Blood
Sciences, Universiti samples were obtained at various postprandial intervals
Putra Malaysia,
43400 UPM, Ser- to assess changes in plasma glucose, cholesterol, and
dang, Selangor, Ma- amylase activity. The results showed that soaking and
laysia autoclaving significantly reduced anti-nutritional fac-
4 Department of Ani-
mal Science, Univer- tors, including tannins, phytic acid, saponins, and tryp-
siti Putra Malaysia, sin inhibitors, without causing major alterations in the
43400 Serdang, Se- basic nutrient composition of CSGM. These treatments
langor, Malaysia
*Corresponding au- also improved the digestibility of dry matter, protein,
thor: Abdalbast H. I. and energy compared with the diet containing untreated
Fadel, CSGM, with soaked CSGM yielding the highest digest-
E-mail addresses: ibility values. Blood metabolites peaked 8 hours after
basitfadel@ya-
hoo.com feeding across all treatments. Fish fed the reference diet
Department of Ma- exhibited higher plasma glucose and cholesterol levels
rine Resources, Omar and lower amylase activity than those fed CSGM-based
Al-Mukhtar Univer-
sity, Al-Bayda, Libya diets. Overall, the findings indicate that pre-treatment of
CSGM enhances digestibility and reduces anti-